Plasmid_Backbone

Part:BBa_K4404011

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-10)


pMTL82151

pMTL82151 is based on the modular plasmid system ClosTron (Heap et al., 2009) with the Gram-positive replicon pBP1 and ColE1 as a Gram-negative replicon. traJ facilitates the transfer of the plasmid and the catP cassette serves as an antibiotic (chloramphenicol) selection marker. lacZ codes for β-galactosidase, sits at the multiple cloning site and therefore serves as the transformation control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 80
    Illegal XbaI site found at 107
    Illegal SpeI site found at 2268
    Illegal PstI site found at 146
    Illegal PstI site found at 5224
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 80
    Illegal NheI site found at 325
    Illegal SpeI site found at 2268
    Illegal PstI site found at 146
    Illegal PstI site found at 5224
    Illegal NotI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 80
    Illegal BglII site found at 134
    Illegal BamHI site found at 101
    Illegal XhoI site found at 138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 80
    Illegal XbaI site found at 107
    Illegal SpeI site found at 2268
    Illegal PstI site found at 146
    Illegal PstI site found at 5224
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 80
    Illegal XbaI site found at 107
    Illegal SpeI site found at 2268
    Illegal PstI site found at 146
    Illegal PstI site found at 5224
    Illegal NgoMIV site found at 2773
    Illegal NgoMIV site found at 4680
    Illegal NgoMIV site found at 4690
    Illegal NgoMIV site found at 4816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 4432
    Illegal SapI.rc site found at 4787
    Illegal SapI.rc site found at 5158


[edit]
Categories
Parameters
None